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ZenBio adipocyte differentiating medium dm-2
Adipocyte Differentiating Medium Dm 2, supplied by ZenBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/adipocyte+differentiation+medium/differentiation+medium+dm+2/10__1016_slash_j__bbrep__2025__102030-61-6-10
Average 90 stars, based on 1 article reviews
adipocyte differentiating medium dm-2 - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Cell Culture:

Article Title: Engineered three-dimensional breast tissue, adipose tissue, and tumor disease model
Article Snippet: Subcutaneous preadipocytes (SPA) were acquired from Zen-Bio (Research Triangle Park, N.C.) and cultured in subcutaneous preadipocyte medium for expansion. .. Three days prior to bioprinting, cells were cultured in adipocyte differentiation medium (Zen-Bio). ..

Article Title: Therapeutic peptides
Article Snippet: Mouse 3T3-L1 cells were seeded at a density between 1,000-20,000 cells per well on 96-well, 48-well, or 24-well plates in Pre-adipocyte Medium (Zen-Bio, Durham, N.C.) and grown to confluence at 37° C. in a humidified atmosphere of 5% CO2/95% air. .. Two days after confluence, cells were placed in Adipocyte Differentiation Medium (Zen-Bio, Durham, N.C.) and cultured for three additional days at 37° C. in a humidified atmosphere of 5% CO2/95% air. .. The culture media was then replaced with Adipocyte Maintenance Medium (Zen-Bio) and the cells were maintained for an additional 9-14 days at 37° C. in a humidified atmosphere of 5% CO2/95% air with partial medium replacement every other day.

other:

Article Title: Therapeutic peptides
Article Snippet: The culture media was then replaced with Adipocyte Maintenance Medium (Zen-Bio) and the cells were maintained for an additional 11-21 days at 37° C. in a humidified atmosphere of 5% CO2/95% air with partial medium replacement every other day.

Article Title: Therapeutic peptides
Article Snippet: The culture media was then replaced with Adipocyte Maintenance Medium (Zen-Bio) and the cells were maintained for an additional 9-14 days at 37° C. in a humidified atmosphere of 5% CO2/95% air with partial medium replacement every other day.

Article Title: Engineered three-dimensional breast tissue, adipose tissue, and tumor disease model
Article Snippet: Slides were then dehydrated through an ethanol gradient, cleared in xylene, and mounted with resinous mounting media (CytoSeal, Fisher Scientific).

Staining:

Article Title: Selectivity matters: selective ROCK2 inhibitor ameliorates established liver fibrosis via targeting inflammation, fibrosis, and metabolism
Article Snippet: .. For human cell adipogenesis induction, cells were plated at confluence for overnight, the following day, cells were fed with adipocyte differentiation medium (DM-2, ZenBio) with or without indicated concentrations of ROCK inhibitors, 7 days after induction, cells were subjected to Oil Red O staining, or collected for RNA extraction, or collected for western analysis. .. For murine 3T3L1 cell adipogenesis, cells were plated at confluence, after two days, cells were fed with differentiation medium (DM-2, ZenBio) with or without indicated concentrations of ROCK inhibitors, incubated for 3 days, then cells were switched to adipocyte maintenance medium (AM-1, ZenBio) with or without indicated concentrations of ROCK inhibitors for 3 more days.

RNA Extraction:

Article Title: Selectivity matters: selective ROCK2 inhibitor ameliorates established liver fibrosis via targeting inflammation, fibrosis, and metabolism
Article Snippet: .. For human cell adipogenesis induction, cells were plated at confluence for overnight, the following day, cells were fed with adipocyte differentiation medium (DM-2, ZenBio) with or without indicated concentrations of ROCK inhibitors, 7 days after induction, cells were subjected to Oil Red O staining, or collected for RNA extraction, or collected for western analysis. .. For murine 3T3L1 cell adipogenesis, cells were plated at confluence, after two days, cells were fed with differentiation medium (DM-2, ZenBio) with or without indicated concentrations of ROCK inhibitors, incubated for 3 days, then cells were switched to adipocyte maintenance medium (AM-1, ZenBio) with or without indicated concentrations of ROCK inhibitors for 3 more days.

Western Blot:

Article Title: Selectivity matters: selective ROCK2 inhibitor ameliorates established liver fibrosis via targeting inflammation, fibrosis, and metabolism
Article Snippet: .. For human cell adipogenesis induction, cells were plated at confluence for overnight, the following day, cells were fed with adipocyte differentiation medium (DM-2, ZenBio) with or without indicated concentrations of ROCK inhibitors, 7 days after induction, cells were subjected to Oil Red O staining, or collected for RNA extraction, or collected for western analysis. .. For murine 3T3L1 cell adipogenesis, cells were plated at confluence, after two days, cells were fed with differentiation medium (DM-2, ZenBio) with or without indicated concentrations of ROCK inhibitors, incubated for 3 days, then cells were switched to adipocyte maintenance medium (AM-1, ZenBio) with or without indicated concentrations of ROCK inhibitors for 3 more days.



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Single-cell RNA sequencing profiling of joint cell populations following extracellular vesicle (EVs) treatment. (a) The UMAP plot displaying the classification of joint cell populations into ten distinct clusters under three conditions: WT, PBS-treated (PBS), and EVs-treated (EVs). ( b ) Cell typing of clusters based on gene expression. Cluster 1 (NK cells), Cluster 2 (T cells), Cluster 3 (pre-B cells), Cluster 4 (Neutrophils), Cluster 5 (B cells), Cluster 6 (Chondrocyte progenitor cells), Cluster 7 (Hematopoietic progenitors), Cluster 8 <t>(Mesenchymal</t> cells), Cluster 9 (Plasma cells), and Cluster 10 (Erythroblasts). ( c ) Heatmap displaying the expression of representative marker genes for each cluster, including Col12a1 for the
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Average 90 stars, based on 1 article reviews
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  Buy from Supplier

Image Search Results


Single-cell RNA sequencing profiling of joint cell populations following extracellular vesicle (EVs) treatment. (a) The UMAP plot displaying the classification of joint cell populations into ten distinct clusters under three conditions: WT, PBS-treated (PBS), and EVs-treated (EVs). ( b ) Cell typing of clusters based on gene expression. Cluster 1 (NK cells), Cluster 2 (T cells), Cluster 3 (pre-B cells), Cluster 4 (Neutrophils), Cluster 5 (B cells), Cluster 6 (Chondrocyte progenitor cells), Cluster 7 (Hematopoietic progenitors), Cluster 8 (Mesenchymal cells), Cluster 9 (Plasma cells), and Cluster 10 (Erythroblasts). ( c ) Heatmap displaying the expression of representative marker genes for each cluster, including Col12a1 for the

Journal: Regenerative Therapy

Article Title: Extracellular vesicles derived from adipose-derived mesenchymal stem/stromal cells prevent synovial inflammation and attenuate cartilage degeneration in rodent osteoarthritis

doi: 10.1016/j.reth.2025.101056

Figure Lengend Snippet: Single-cell RNA sequencing profiling of joint cell populations following extracellular vesicle (EVs) treatment. (a) The UMAP plot displaying the classification of joint cell populations into ten distinct clusters under three conditions: WT, PBS-treated (PBS), and EVs-treated (EVs). ( b ) Cell typing of clusters based on gene expression. Cluster 1 (NK cells), Cluster 2 (T cells), Cluster 3 (pre-B cells), Cluster 4 (Neutrophils), Cluster 5 (B cells), Cluster 6 (Chondrocyte progenitor cells), Cluster 7 (Hematopoietic progenitors), Cluster 8 (Mesenchymal cells), Cluster 9 (Plasma cells), and Cluster 10 (Erythroblasts). ( c ) Heatmap displaying the expression of representative marker genes for each cluster, including Col12a1 for the "Chondrocyte" cluster, and Col1a1 for the "Mesenchymal cell" cluster, and CD45/CD14 for the "Hematopoietic" cluster.

Article Snippet: For adipocyte differentiation, either BioMirai Lab's induction kit or PromoCell's mesenchymal stem cell adipocyte differentiation medium was used, with Oil Red O staining to assess lipid accumulation.

Techniques: RNA Sequencing, Gene Expression, Clinical Proteomics, Expressing, Marker

Differential gene expression besed on Single-cell RNA sequencing of joint cell clusters following extracellular vesicle (EVs) treatment . ( a-d ) Comparison of gene expression in EVs, PBS, and WT groups (a) in Cluster 6 (chondrocyte progenitors) (COL2A1, COL1A2, PRG4, MMP3, CCL2, and FGF18). ( b ) in Cluster 1 (NK cells) (CD14, MRC1, CD163, CD86, CD80, and NOS2). ( c ) in Cluster 8 (Mesenchymal cells) (COL2A1, COL1A2, PRG4, CCL2, and FGF18) ( d ) in Cluster 4 (Neutrophils) (APOE, AGPAT4, HAPLN1, CDKN1C, MET, and CEMIP2). (e) Flow cytometry analysis confirmed that the proportion of CD11b + CD163 + M2 macrophages was approximately 2.5-fold higher in the EVs-treated group compared to the PBS-treated group.

Journal: Regenerative Therapy

Article Title: Extracellular vesicles derived from adipose-derived mesenchymal stem/stromal cells prevent synovial inflammation and attenuate cartilage degeneration in rodent osteoarthritis

doi: 10.1016/j.reth.2025.101056

Figure Lengend Snippet: Differential gene expression besed on Single-cell RNA sequencing of joint cell clusters following extracellular vesicle (EVs) treatment . ( a-d ) Comparison of gene expression in EVs, PBS, and WT groups (a) in Cluster 6 (chondrocyte progenitors) (COL2A1, COL1A2, PRG4, MMP3, CCL2, and FGF18). ( b ) in Cluster 1 (NK cells) (CD14, MRC1, CD163, CD86, CD80, and NOS2). ( c ) in Cluster 8 (Mesenchymal cells) (COL2A1, COL1A2, PRG4, CCL2, and FGF18) ( d ) in Cluster 4 (Neutrophils) (APOE, AGPAT4, HAPLN1, CDKN1C, MET, and CEMIP2). (e) Flow cytometry analysis confirmed that the proportion of CD11b + CD163 + M2 macrophages was approximately 2.5-fold higher in the EVs-treated group compared to the PBS-treated group.

Article Snippet: For adipocyte differentiation, either BioMirai Lab's induction kit or PromoCell's mesenchymal stem cell adipocyte differentiation medium was used, with Oil Red O staining to assess lipid accumulation.

Techniques: Gene Expression, RNA Sequencing, Comparison, Flow Cytometry